Microencapsulation and Fermentation of Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12
Microencapsulation and Fermentation of Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12
Blog Article
Because of poor survival of probiotic bacteria, microencapsulation evolved from the immobilized cell culture technology here used in the biotechnological industry.Two probiotic strains, Bifidobacterium (BB-12) and Lactobacillus acidophilus (LA-5) were immobilized in calcium alginate by extrusion method.Encapsulation parameters and efficacy of this method were evaluated.Growth factors of these two bacteria were also measured by culturing in 10-L fermenter.Growth tillman 750m curves were obtained with respect to optical density and dry biomass weight.
Encapsulation yield was over than 60% in each experiment.Scanning electron microscopy (SEM) of Entrapment of cells in alginate matrix and cross-sections of dried bead were obtained and illustrated.Bifidobacterium have been shown better biotechnological properties.